SCIENCE TOOLS

• PUBMED

• GOOGLE SCHOLAR

• PROTEIN DATA BANK (PDB)

• BLAST

• HMMER

• SEQUENCE ALIGNMENT SOFTWARE

• CLUSTAL OMEGA (PROTEIN ALIGNMENT)

• CLUSTALW

• MULTIPLE ALIGN SHOW

• ESPRIPT 3.0

• HHPRED

• PHYRE2

• RAPTOR

• PSIPRED

• DISPROT (PROTEIN DIOSRDER)

• COACH-D (PROTEIN-LIGAND BINDING SITE PREDICTION)

• trROSETTA

• SMART

• EXPASY

• BIP SERVER

• PHOBIUS (COMBINED TOPOLOGY AND SIGNAL PEPTIDE PREDICTOR)

 

REDOX TOOLS

• REDOXDB

• CYSREDOX

• DSDBASE - DISULFIDE DATABASE

• CYSMODDB

• iCYSMOD

• METOSITE

CORNELL FACILITIES

• E-SHOP

• DNA SEQUENCING FACILITY

  • HOW TO READ A SEQUENCE CHROMATOGRAM

  • SNAPGENE (VIEW CHROMATOGRAMS)

  • SOFTWARE FOR CHROMATOGRAM VIEWING (4PEAKS)

  • SOFTWARE FOR CHROMATOGRAM VIEWING AND SEQUENCE ALIGNMENT (APE)

• PROTEOMICS CORE

• TREx FACILITY (RNASEQ)

YEAST RESOURCES

• SACCHAROMYCES GENOME DATABASE

• YEAST RESOURCE CENTER

• HORIZON (YEAST STRAINS FOR PURCHASE)

• THERMOFISHER (YEAST STRAINS FOR PURCHASE)

• PRIMERS-4-YEAST (SCHULDINER LAB)

• INTRODUCTION TO YEAST GENETICS AND MOLECULAR BIOLOGY

• FRED SHERMAN'S GETTING STARTED WITH YEAST

• COMMON LAB SACCHAROMYCES CEREVISIAE STRAINS

• COMMON SACCHAROMYCES CEREVISIAE AUXOTROPHIC MARKER MUTATIONS

• CORA STYLE'S YEAST IDIOSYNCRASIES
   

MOLECULAR BIOLOGY AND BIOCHEMISTRY RESOURCES

• THE CELL MAP

• FITDB

• YEASTMINE

• LOQATE

• SCPD (PROMOTER DATABASE)

• YEASTRACT (TF BINDING SITES)

• GWIPS (GENOME BROWSER)

• GUIDE TO MOLECULAR CLONING (ADDGENE)

• PLASMIDS 101 (ADDGENE)

• QIAGEN MINIPREP HANDBOOK

• QIAGEN QIAQUICK HANDBOOK

    MUTAGENESIS MANUALS:

• AGILENT QUIKCHANGE MANUAL (BASIS FOR LAB QC PROTOCOL)

• AGILENT QUIKCHANGE MULTI MANUAL (BASIS FOR LAB MULTI-QC PROTOCOL)

• AGILENT QUIKCHANGE MULTI LIGHTNING MANUAL (PURCHASED KIT)

• QUIKCHANGE OLIGO DESIGN WEBSITE

• QUIKCHANGE PRIMER MELTING TEMPERATURE CALCULATOR

   BACTERIAL STRAIN INFO:

• COMMON LAB BACTERIA GENOTYPES (CLONING AND PROTEIN EXPRESSION)

• COMMON LAB BACTERIA GENTOYPES (FOCUSED ON STRAINS FOR CLONING)

• BACTERIAL STRAINS FOR PROTEIN EXPRESSION (ADDGENE LIST - ALSO LISTS BACKGROUND INFO.)

• BACTERIAL STRAINS FOR PROTEIN EXPRESSION (WOLFSON CENTER LIST)

 

   NEW ENGLAND BIOLABS STUFF:

• NEB RESTRICTION ENZYME BUFFER CHART (OLD BUFFER SYSTEM)

• NEB RESTRICTION ENZYME BUFFER CHART (NEW BUFFER SYSTEM)

• INTERACTIVE DOUBLE DIGEST BUFFER FINDER (NEW BUFFER SYSTEM)

• INTERACTIVE ENZYME FINDER

• HOW WELL DOES YOUR RESTRICTION ENZYME CUT AT DNA ENDS ?

• ISOSCHIZOMERS

• COMPATIBLE COHESIVE ENDS

• PHUSION ENZYME

• TAQ ENZYME

• 1 KB DNA LADDER

   EXPRESSION PLASMID INFO:

• pET E. COLI T7 EXPRESSION VECTORS

• NOVAGEN pET MANUAL

• pGEX E. COLI EXPRESSION VECTORS

   GE HEALTHCARE (CYTIVA) PROTEIN PURIFICATION HANDBOOKS:

• DOWNLOAD LINK (2022 VERSIONS)

   THERMO SCIENTIFIC PIERCE TECHNICAL HANDBOOKS:

• DIALYSIS, DESALTING, DETERGENT REMOVAL

• PROTEIN ASSAYS (INCLUDES COMPATIBILITY TABLE)

• AVIDIN-BIOTIN

• METHODS TO MONITOR PROTEIN INTERACTIONS

   ADDITIONAL HANDBOOKS / TABLES:

• QIAGEN NI-NTA HANDBOOK

• CALBIOCHEM'S DETERGENT BOOK I

• CALBIOCHEM'S DETERGENT BOOK II

• ROCHE PROTEASE INHIBITORS HANDBOOK

• CALBIOCHEM'S INHIBITORS HANDBOOK

• BUFFER TABLES

• pH VERSUS TEMPERATURE FOR TRIS (A GUIDELINE - CHECK ACTUAL pH WITH METER)

• ANTIBODY COMPATIBILITY WITH PROTEIN A / PROTEIN G

GENERAL LAB RESOURCES

• LAB MATH

• ROTOR RPM <-> RCF CONVERSION

• WILEY CURRENT PROTOCOLS

• MOLECULAR PROBES HANDBOOK

• SOME BASICS ON HOW TO KEEP A LAB NOTEBOOK

• CHEMICAL RESISTANCE OF PLASTICS